Day 1 :
Slovak Society for Aesthetic & Cosmetic Dermatology, Slovakia
Keynote: Carboxytherapy – non-invasive method in dermatology, aesthetic dermatology and some other branches of medicine
Time : 10:00-10:40
Zelenkova Hana has been active in the field of Dermatovenerology since 1973. Since 2000 she has been directing her own Private Clinic of Dermatovenereology. She is a Coordinator of many international multicentre trials. She has 420 scientific publications to her credit and is the author of the book “Carboxytherapy” (2015), translated into 4 languages. She is regularly invited to deliver lectures at international congresses and she is the Founder and President of the Slovak Society for Aesthetic and Cosmetic Dermatology (SSEDK), Organizer and President of the Traditional International Dermaparty Congress. She is the Vice-President European Society of Aesthetic and Cosmetic Dermatology since 2006 and President of the European Society of Aesthetic and Cosmetic Dermatology since 2007.
Carboxytherapy; therapeutically applied carbon dioxide injections have been used in balneotherapy since 1932. This treatment modality has become the centre of attention as a unique method applicable in dermatology, aesthetic dermatology and anti-aging medicine. This unique method is minimum invasive and non-aggressive which is comfortable for the patient and producing excellent effects without the risk of undesired side effects. In aesthetic dermatology this method may be applied as a rejuvenation modality and is employed mainly due to its classic vasodilatation effect and its capacity to foster intradermal collagen restructuring. In classic dermatology it is used to treat patients with poor healing lower leg ulcers, in diabetic patients and in patients with poor healing surgical wounds. Carboxytherapy is efficient also in some psoriasis manifestations when combined with some other traditional approaches, in circumscript scleroderma, lichen verrucosus as well as hair loss. In aesthetic dermatology the effect is manifest quite soon (usually after two sessions already in the course of 7-14 days). It significantly and visibly improves the tonus of the skin as well as other aesthetic parameters (especially while treating skin laxity in abdomen area, inner arms and thighs and double chin or saggy eyelids. Good effects also show following the application of carboxytherapy to treat stretch marks, cellulite and scars (also older and more extensive scars after burns). Great effects are achieved in correcting the side effects of ill performed interventions such as liposuction or in using carboxytherapy directly to shape problem body areas (thighs, hips and abdomen).
Mercer University, USA
Time : 10:50-11:30
Ajay K Banga is a Professor and Department Chair in the Department of Pharmaceutical Sciences at the College of Pharmacy, Mercer University, USA. He also holds an Endowed Chair in transdermal delivery systems and serves as Co-Director for the Center for Drug Delivery Research. He has completed his PhD in Pharmaceutical Sciences from Rutgers University, NJ. He has over 300 publications and scientific abstracts to his credit. He currently serves on the Editorial Board of 10 journals, Associate Editor for one journal and has served as the Editor-in-Chief for a Drug Delivery journal. He has written three single author books and over 12 book chapters in the areas of transdermal delivery and protein formulation/delivery. He is a Fellow of the American Association of Pharmaceutical Scientists.
This presentation will discuss drug-device combinations to enhance dermal delivery of cosmeceuticals and pharmaceuticals. We have recently shown that physical treatments by ablative laser and polymeric microneedles enhanced skin delivery of methotrexate and other actives. Poly (D, L-lactide-co-glycolide) microneedles, fabricated by melting technique and ablative laser (2940 nm) were both able to significantly enhance delivery. Successful skin poration by microneedles and ablative laser were demonstrated by dye binding, histology and DermaScan studies. Dimensions of microchannels in skin were measured by Scanning Electron Microscopy, pore uniformity and confocal laser microscopy. The feasibility of transdermal delivery of human growth hormone through laser-microporated skin was also demonstrated and the effect of depth and density of micropores was studied. Recently, we have used in vitro microdialysis as a promising technique to quantify lateral diffusion of drugs in skin. The rate of lateral diffusion of Diclofenac sodium was enhanced by microneedle and ablative laser treatment. Other technologies of interest include iontophoresis and phonophoresis. Recent innovations in these technologies will be presented. We have demonstrated iontophoretic delivery of several drug molecules including several polypeptides.
Mercer University, USA
Keynote: Microneedle based transdermal vaccines for infectious diseases and cancer- Are we there yet?
Time : 11:30-12:10
Martin J D Souza has obtained his PhD degree from the University of Pittsburgh, PA, USA. He is working as a Professor and Director of Graduate Programs in the College of Pharmacy at Mercer University in Atlanta, Georgia. He also serves as the Director of the Clinical Laboratory and Co-Director of the Center for Drug Delivery Research. He has published over 90 manuscripts and has been the recipient of several research grants from the National Institutes of Health (NIH), the American Diabetes Association, the Georgia Cancer Coalition and the Georgia Research Alliance. He serves on several Editorial Boards and is a journal Reviewer for over 10 scientific journals and has several patents issued in the area of Nanotechnology.
Our Vaccine Nanotechnology Laboratory at Mercer University has been working on the design and delivery of microparticulate vaccines for both infectious diseases and cancer. In this presentation, we discuss the triumphs and tribulations of this rather innovative, in-expensive and painless method of vaccine delivery. Our patented technology is rather broad based and can be used to administer multiple vaccines in a single set of micro-needles. On the infectious disease vaccine front, we have been working on the vaccines for several infectious diseases such as influenza, HPV, RSV, meningitis, gonorrhea and measles vaccines designed for delivery via microneedles. In this presentation, we will discuss the results of some of these vaccine prototypes. The vaccine antigen was formulated in bio-degradable and bio-compatible matrices to prepare microparticles. These vaccine microparticles were administered using micro-needles via the transdermal route. Serum samples were obtained at regular intervals in-order to determine the antigen specific antibody responses (such as IgG). Animals were challenged with live virus/bacteria to determine the level of protective immunity developed after vaccination. Further, we examined the expression of co-stimulatory molecules that impact antigen presentation in human macrophages pulsed with the antigen. We also evaluated antigen presentation (CD80) and death signal (CD 95) in an in vitro setup where antigen-presenting cells (APCs) primed by the antigen were used to stimulates plenocytes that had never been exposed to the antigen. The up-regulation of other co-stimulatory molecules such as CD-40, CD-80 and CD-86 were also determined. In conclusion, the novel vaccine particles are robustly taken up by macrophages and up-regulate co-stimulatory molecules that enhance antigen presentation which is a pre-requisite for inducing adaptive immunity. The other innovative microneedle particulate vaccines under study are therapeutic cancer vaccines such as breast, melanoma, ovarian and prostate cancer vaccines. The microneedle formulations resulted in reduced tumor growth. We also report on the effect of adjuvants such as Alum, MF-59 and MPL on enhancing the potency of these vaccine proto-types. Microparticulate vaccine was prepared by entrapping tumor-associated antigens (TAAs), in a polymer matrix of albumin and EUDRAGIT polymers using a Buchi mini spray dryer. Animals were exposed to tumor cell and once the tumor was palpable, these animals received the vaccine microparticles as prime and boosters via transdermal route through microneedles. The tumor growth was routinely monitored. Mechanistic studies such as natural killer cell activity, CD8+ and CD4+ T-Lymphocyte activity after vaccination were also carried out in order to study the mechanism by which the vaccine works to modulate immune response. Flow cytometric analyses for CD8 and CD4 T-cell assays, NK-cell activity were carried out to assess vaccine efficacy. Based upon the vaccine response data, the tumor retardation was found to be significant after transdermal administration. Vaccination may prove to be an efficient treatment for cancer patients in the future.
- Dermatological procedure | Diagnostic procedure and Treatment | Dermatological skin diseases
University of Sao Paulo, Brazil
Gulhane Military Medical Academy, Turkey
University of Sao Paulo, Brazil
Title: RTKs and Transcription Factors Proteins Analysis in a serie of Head and Neck Mucosal Melanoma
Time : 12:10-12:40
Ricardo Hsieh was graduated from the Department of Dermatology, Medical School of University of Sao Paulo and obtained his Master’s and PhD degree. He is currently a Post-doctoral fellow at Department of Pathology, Dental School and Associate Research Scientist at Institute of Tropical Medicine.
Head and Neck Mucosal Melanoma (HNMM) is rare, accounting 1% of all melanomas in the USA and 6% in Japan. It is known that receptor tyrosine kinase (RTK) signaling mediates the development and progression of melanoma; its development is a complex process involving activation of proto-oncogenes and loss of tumor suppressors. The MERTK, AXL and TYRO3 functionally engage in cooperative or distinct signaling cascade and there is evidence that they play a key role in melanoma pathogenesis. C-kit plays a key role in melanocytic development, migration and proliferation; its mutations seem to be more common in mucosal and acral melanomas. Moreover, Bcl-2 oncogene encodes a family of anti-apoptotic proteins and it is overexpressed in melanomas, proto-oncogene C-myc is a transcriptional factor and plays crucial role both in driving cell proliferation and promoting apoptosis, its overexpression has been associated to melanoma progression and oncogene SOX10 cooperates with other transcription factors to direct the development and differentiation of melanocytes. These three nuclear markers are associated to melanoma’s metastatic risk. We studied MERTK, AXL, TYRO3, BCL-2, C-myc and SOX10 in 29 FFPE Head and Neck cases organized in TMA by Alkalin Phosphatase Immunohistochemistry technique. We observed positive expression in: 4/29 MERTK, 29/29 AXL and TYRO3, 15/29 CKIT, 27/29 Bcl-2, 28/29 SOX10, 16/29 C-myc. According to our results, loss of MERTK and CKIT proteins expression seems to play an important role in the HNMM pathogenesis and BCL-2 and SOX10 should be good adjunctive biomarkers for HNMM, further molecular biology studies should corroborate this present study.
Tehran University of Medical Sciences, Tehran, Iran
Title: Obvious synergistic effect of fractional CO2 laser before hair grafting on repigmentation in the treatment of refractory and stable vitiligo: A randomized prospective half lesion comparative study
Time : 12:40-13:10
Amir Feily is dermatologist and researcher in Skin and Stem Cell research Center of Tehran University of Medical Sciences, Tehran and Department of Dermatology of Jahrom University of Medical Sciences, Jahrom, Iran. He is the member of International Society of Dematology , European Academy of Dermatology, Editorial Board of Journal Dermatology Report, Journal of Pigmentary disorder, Aperito Journal of Dermatology , Journal of Drug Metabolism and Toxicology, International Journal of Dermatology Research and Therapy and Associated editor of Asian Journal of Dermatology. He is also the reviewer of many dermatologic journals such as British Journal of Dermatology (BJD), International Journal of Dermatology (IJD),Clinical Experimental dermatology(CED), Journal of European Academy of Dermatology (JEADV) and etc. He has More than 74 high quality papers in Dermatology and Pharmacology. His recent two awards were 2013 Global Education Award, granted by International Society of Dermatology (IJD) and Imrich Sarkani Non-European Memorial award Granted By European Academy of Dermatology at 23rd EADV Congress Amsterdam, the Netherland 2014.
Background: Fractional CO2 laser has been reported to be effective in the treatment of refractory and stable vitiligo.
Objectives: Our purpose was to evaluate the effect of the fractional CO2 laser followed by follicular isolation hair transplantation in the treatment of refractory and stable vitiligo and comparing it with hair transplant alone.
Methods: A total of 20 patients with refractory and stable vitiligo presenting at our clinic were enrolled in this randomized prospective half lesion comparative study. Several resistant patches were selected in every patient and randomly divided to two parts and just one part (Part A) was treated with one session fractional CO2 laser. After 5 days equal number of black graft were harvested from scalp and transplanted in both parts. After 5 days dressing the recipient areas were exposed to NBUVB phototherapy twice a week for 3 months. The diameter of repigmentation around each graft was measured monthly by caliper.
Results: After 3 months of examination perifollicular repigmentation was detectable surrounding the 74% of grafted hair follicles. At the end of the first, second and third months of examination the diameter of perifollicular repigmentation of part A was more than part B with 2.96 ± 0.7 mm relative to 2.51 ± 0.6 mm in the first month, 5.36 ± 4.6 mm relative to 3.59 ± 1 mm in the second month and 6.6 ± 5.8 mm relative to 4.3 ±1.8 mm in the third month. There was statistically significant difference between the two groups after all three months of examination.
Conclusion: This study suggests that fractional CO2 laser therapy followed by hair transplant alongside NBUVB photo therapy could be used effectively as an alternative modality for the treatment of refractory vitiligo. Despite promising results, additional studies are required to confirm our study.
Gulhane Military Medical Academy, Turkey
Time : 14:00-14:30
Ercan Arca was graduated from Gulhane Medical Academy, Faculty of Medicine in 1990. He has completed his Residency during 1993-1996 at Department of Dermatology. He has been working in GMMA, School of Medicine, Department of Dermatology since 2000. He is currently a Professor practicing in the same department. He is interested in psoriasis, biologic agents, pediatric dermatology, cutaneous surgery, laser and cosmetic surgery such as chemical peelings, laser epilation, botox, fillers, facial rejuvenation, dermoscopy, dermatopathology, cutaneous lymphoma. He has published more than 100 papers in reputed journals and also presented 200 oral and poster presentation in both national and international congresses.
Basal cell carcinoma (BCC) is the most common of all cutaneous malignancy. It is locally invasive and often grows slowly. Dermoscopy is a non-invasive, in vivo technique that greatly enhances the clinical diagnostic accuracy in pigmented and non-pigmented skin lesions. Up-to date, dermoscopy has been shown to increase BCC diagnosis by enhancing the differentiation from other skin tumors and inflammatory skin diseases. Classical dermoscopy algorithm for the diagnosis of BCC, especially for the pigmented variant, includes the absence of pigmented network and the presence of at least one of the following criteria: Multiple blue/gray ovoid nests, spoke-wheel structures, arborizing vessels and ulceration. However, BCC may reveal a large variety of dermoscopic features. These non-classical features include some additional criteria more frequently seen in superficial BCC or Fibroepithelioma of Pinkus such as short-fine superficial telangiectasia, concentric structures, multiple small erosions, multiple in focus blue/gray dots, shiny white-red structure less areas. Some dermoscopic structures also present are just visible with polarized non-contact dermoscopy like shiny-white lines (chrysalis) and rosetas. In this presentation, a summary of the dermoscopic features of different types of BCCs and also the value of dermoscopy for diagnosis and management of BCCs will be discussed.
Celal Bayar University, Turkey
Title: The dermoscopic monitoring of targetoid hemosiderotic haemangioma, also known as hobnail haemangioma
Time : 14:30-15:00
Mustafa Turhan Sahin has completed his Medical Education from Erciyes University Medical Faculty, Turkey in 1989. His Dermatology Residency was in Dokuz Eylül University Medical Faculty, Turkey. He is currently working as a Professor in the Celal Bayar University, Medical Faculty Department of Dermatology, Turkey. Many of his studies and case reports were published in national and international journals. He is a Member of Dermatovenereology Association of Turkey, Turkish Dermatology Association, Turkish Dermatopathology Association, Turkish Skin and Venereologic Diseases Association, International Society of Dermatology (ISD), European Academy of Dermatology and Venereology (EADV), American Academy of Dermatology (AAD) (Since 2001) and also a Board Member in International Dermoscopy Society (IDS).
Targetoid hemosiderotic hemangioma, also known as hobnail haemangioma, is benign, solitary, targetoid vascular neoplasm occurring predominantly on the proximal extremities and trunk. Most patients exhibit a typical, targetoid haemangioma, whereby a small, solitary, purple and/or brown papule is surrounded by an ecchymotic ring that can expand or subsequently disappear with persistence of the central papule. The halo, however, is not a constant finding, thus the term hobnail haemangioma was coined to describe the non-targetoid variant of targetoid hemosiderotic haemangioma. Duration of the lesions ranges from 1 month to 20 years. During this time, targetoid hemosiderotic haemangioma may undergo episodic and cyclic changes in morphology. Dermoscopy has been scarcely used in the diagnosis of targetoid hemosiderotic haemangioma. Here we describe our cases of targetoid hemosiderotic haemangioma and study their monthly changing dermoscopical findings to show that this technique could be useful in the monitoring and differential diagnosis of this scarcely reported entity.
University of Barcelona, Spain
Title: Nanotechnology Design & Development of Antimicrobial Ingredients for Deep Skin Acne Treatment
Time : 15:00-15:30
Camila Folle was graduated in Pharmaceutical Science from London Metropolitan University in 2011 and has completed Masters degree in Cosmetics & Dermopharmacy by CESIF Barcelona, including a year Internship at ISDIN S.A., and recently completed second Master’s degree in Nanoscience & Nanotechnology (2015) from the University of Barcelona. Presently, she is researching dermatological active ingredients applied nanotechnology.
Antimicrobial nanotechnology show promising activity for topical delivery on dermatologic acne treatment due to its potential of being mainly retained in the deeper layers of the skin, where acne develops. The acne evolution occurs by excessive sebum production at the hair follicle proximities, which leads to Propionibacterium acnes proliferation and a resulting inflammation. It is relevant that nanotechnology has several advantages such as, small particle diameter to penetrate inside skin tissues, sustained drug withdrawn and lower doses required for its efficacy. Moreover, the skin fat tissues are likely to behave as a deep hosting compartment for the nanoparticles. This research relies primarily on optimization and characterization of the desired formulation, then determining the efficacy of the drug nanosystem against P. acne by its minimal inhibitory concentration (MIC). Thus, in vitro release profiles of the drug nanoparticles and the free drug are compared under kinetic models, determining its biopharmaceutical transmembrane behavior using Franz diffusion cell. The skin permeability parameters are studied in ex vivo models, determining the amounts of drug retained inside the skin, using skin extraction and tape stripping techniques. P. acnes is also hosted by deep skin tissues, therefore, the amount of drug found retained in the deeper layers of the skin is then calculated towards the MIC value of the antimicrobial agent and further classified its efficiency for acne treatment. Nevertheless, this advanced methodology exempts the use of animal test, being suitable for cosmetic industries.
- Dermatological Hair Problems
Jahrom University of Medical Sciences, Jahrom, Iran
Title: Workshop on Feily’s method as new mode of hair grafting in prevention of scalp necrosis even in dense hair transplantation
Time : 15:40-17:30
Amir Feily is dermatologist and researcher in Skin and Stem Cell research Center of Tehran University of Medical Sciences, Tehran and Department of Dermatology of Jahrom University of Medical Sciences, Jahrom, Iran. He is the member of International Society of Dematology , European Academy of Dermatology, Editorial Board of Journal Dermatology Report, Journal of Pigmentary disorder , Aperito Journal of Dermatology , Journal of Drug Metabolism and Toxicology, International Journal of Dermatology Research and Therapy and Associated editor of Asian Journal of Dermatology. He is also the reviewer of many dermatologic journals such as British Journal of Dermatology (BJD), International Journal of Dermatology (IJD),Clinical Experimental dermatology(CED), Journal of European Academy of Dermatology (JEADV) and etc. He has More than 74 high quality papers in Dermatology and Pharmacology. His recent two awards were 2013 Global Education Award, granted by International Society of Dermatology (IJD) and Imrich Sarkani Non-European Memorial award Granted By European Academy of Dermatology at 23rd EADV Congress Amsterdam, the Netherland 2014.
Hair restoration is a safe procedure and most of its associated complications are preventable by the surgeon and/or the patient. Recipient area necrosis is rare but arises when an increased number of recipient grafts are utilized and de-vascularization of the scalp occurs. The aim of this study was to investigate and compare all cases and pictures reported in main search engines and Iranian centers of hair transplant to find the dangerous zone of necrosis and to provide a new method for prevention of necrosis. Pictorial analysis of this study revealed that the majority of necrosis (14 of 18) occurs in central region of the scalp and is inclined, particularly, to the right parietal aspect of the scalp. Accordingly, a case series was done and a new method for prevention of scalp necrosis even in dense packing transplantation was discussed.